Nucleic Acids | | | |
Service | Platform | Cost per Sample | |
Nucleic Acid Isolation (RNA or DNA) | KingFisher Flex | $5 | |
Nucleic Acid Isolation (RNA or DNA) | Column | $10 | |
Nucleic Acid Isolation (RNA or DNA) | FFPE | $10 | |
QC Fragment Analysis (RNA, DNA, or pooled libraries) and Concentration Check | QIAxcel/Nanodrop | $5 | |
Fluorescent Nucleic Acid Quantitation (RNA, DNA, or pooled libraries) | Qubit | $2 | |
Total Nucleic Acid Service (Isolation, QC, and quantitation) | All of above instruments | $10 | |
Covaris Sonication | M220 | free with training | |
|
Genotyping |
Service | Platform | Cost per Sample | |
Taqman (one-time charge for primers) | CFX96/CFX384 | $3 | |
PCR-based (one-time charge for primers) | QIAxcel | $3 | |
|
Real-time qPCR, Bio-Rad CFX-96/-384 |
Service | Platform | Cost per Sample | |
qRT-PCR (one-time charge for primers) | CFX96/CFX384 | $2 | |
|
Capillary Sequencing - SeqStudio | |
Service | Cost | | |
Full Service Sequencing (plasmid or PCR product) - per sample | $5 | | |
Full Service Sequencing (plasmid or PCR product) - 96-well plate | $400 | | |
Ready-to-Load Sequencing - 96-well plate | $100 | | |
Fragment Analysis (Ready-to-Load) - 96-well plate | $100 | | |
|
NGS-Based Pipelines |
Service | Nucleic Acid Input | Platform | Cost per Sample |
TruSeq Stranded RNA (Total) | Total RNA | Illumina NextSeq | $200 |
TruSeq Stranded RNA-mRNA | Total RNA | Illumina NextSeq | $150 |
TruSeq Small RNA | Small or Total RNA | Illumina MiSeq or NextSeq | $180 |
TruSeq DNA PCR-free (WGS) | Genomic DNA | Illumina NextSeq | $180 |
TruSeq DNA Methylation | Genomic DNA | Illumina MiSeq or NextSeq | $150 |
ScriptSeq Bacterial RNA | Total RNA | Illumina MiSeq or NextSeq | $125 |
Nextera XT DNA (Small Genomes, Amplicons, Plasmids) | DNA, PCR products, Amplicons | Illumina MiSeq or NextSeq | $50 |
16S Metagenomics | Microbial DNA | Illumina MiSeq or NextSeq | $25 |
Illumina iseq 100 Sequencing (up to 4 million reads) |
Service | Kit Cost | Per Run Charge | Total |
iSeq 100 i1 Reagent v2 (300 cycle) | $874 | $155 | $1,029 |
|
Illumina MiSeq Sequencing (15-25 million reads) |
Service | Kit Cost | Per Run Charge | Total |
MiSeq Reagent Kit v2- 50 cycle (up to 75 cycles or 26x26 PE reads) | $1,083 | $155 | $1,238 |
MiSeq Reagent Kit v3- 150 cycle (up to 175 cycles or 76x76 PE reads) | $1,185 | $155 | $1,340 |
MiSeq Reagent Kit v2- 300 cycle (up to 325 cycles or 151x151 PE reads) | $1,284 | $155 | $1,439 |
MiSeq Reagent Kit v2- 500 cycle (up to 525 cycles or 251x251 PE reads) | $1,493 | $155 | $1,648 |
MiSeq Reagent Kit v3- 600 cycle (up to 625 cycles or 301x301 PE reads) | $1,898 | $155 | $2,053 |
|
Illumina NextSeq 500 Sequencing (150-400 million reads) |
Service | Kit Cost | Per Run Charge | Total |
NextSeq 500/550 Mid Output Kit-150 cycle (75x75 PE cycles) | $1,385 | $285 | $1,670 |
NextSeq 500/550 Mid Output Kit-300 cycle (150x150 PE cycles) | $2,139 | $285 | $2,424 |
NextSeq 500/550 High Output Kit-75 cycle (75 cycles) | $1,841 | $285 | $2,126 |
NextSeq 500/550 High Output Kit-150 cycle (75x75 PE cycles) | $3,389 | $285 | $3,674 |
NextSeq 500/550 High Output Kit-300 cycle (150x150 PE cycles) | $5,342 | $285 | $5,627 |
Illumina NextSeq 2000 Sequencing (100 million - 1.1 billion reads) |
Service | Kit Cost | Per Run Charge | Total |
NextSeq 1000/2000 P1 Reagents (300 Cycles) | $1,400 | $350 | $1,750 |
NextSeq 1000/2000 P2 Reagents (100 Cycles) v3 | $1,606 | $350 | $1,956 |
NextSeq 1000/2000 P2 Reagents (200 Cycles) v3 | $2,887 | $350 | $3,237 |
NextSeq 1000/2000 P2 Reagents (300 Cycles) v3 | $3,779 | $350 | $4,129 |
NextSeq 2000 P3 Reagents (50 Cycles) | $2,456 | $350 | $2,806 |
NextSeq 2000 P3 Reagents (100 Cycles) | $3,481 | $350 | $3,831 |
NextSeq 2000 P3 Reagents (200 Cycles) | $4,763 | $350 | $5,113 |
NextSeq 2000 P3 Reagents (300 Cycles) | $6,300 | $350 | $6,650 |