The role of the Mitochondria Research Core is to facilitate research into mitochondrial function/dysfunction for local PIs, as needed for publications and for grant proposals. The MRC is not a service facility; rather, it provides expertise for studies of mitochondrial energy transduction and ROS production, along with access to tools. As needed, we work with the PIs and their laboratory personnel to provide protocols and training, assistance during mitochondrial isolations and activity analyses, instruction on interpreting and processing raw data, troubleshooting and streamlining procedures, the preparation of data figures and help with writing manuscripts and grant proposals. We prefer to work with PIs and their labs on a collaborative basis, although smaller or preliminary investigations do not require this.
The MRC is currently moving to the 1st floor of the Guyton Research Building, into G130 and G129, from the original location on the 3rd floor of the Old Research Wing, R327, R329 and R333.
The MRC has all equipment for the isolation of mitochondria from animal sources and from yeast, two Oroboros Fluo-Respirometers, a Hitachi U-3000 split-beam UV-Vis spectrometer for scanning and enzyme kinetics, a Cary 8454 diode array UV-Vis spectrometer for rapid acquisition of spectra and enzyme kinetics, an OLIS Clarity diffuse-reflectance UV-Vis spectrometer for obtaining spectra in whole mitochondria and a Molecular Devices plate reader for absorption or fluorescence measurements using 20 or 96 well plates. The MRC also utilizes a new Jasco V-750 UV-visible Spectrophotometer housed in the lab of Dr. Kristin Edwards, a Jasco 5300 90◦ fluorimeter, housed in the lab of Dr. J. Correia, and a Seahorse-24 (Agilent) Flux Analyzer, housed in the lab of Dr. F. Fan (Pharmacology & Toxicology).
The MRC provides protocols, training and facilitation of the following.
The MRC does not charge fees for service. Participating laboratories do contribute to the cost of reagents.