The BD FACSymphony™A3 Cell Analyzer is a novel high parameter system that offers qualitative and quantitative analysis of cells and other particles using multi-parametric analysis. This instrument is configured for five laser (UV 355 nm, Violet 405 nm, Blue 488 nm, Yellow-Green 561 nm, Red 637 nm) analysis and can simultaneously acquire and distinguish between 23 different fluorescent signals in addition to the forward and side scatter parameters allowing for a 25-parameter definition of the cell. The FACSymphony has the capacity to acquire up to 40,000 events per second allowing for a rapid acquisition of samples.Additionally, the BD High-Throughput Sampler (HTS) option can be used to acquire samples from a 96 or 384-well plate. The FACSymphony is operated using FACSDiva 9.1v software. FCS file versions 2.0, 3.0, and 3.1 can be exported for data analysis.