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RIA and Elisa

Current RIA

RadioimmunoassaysSample volume required (ml)Anticoagulants for plasma samples
Angiotensin IExactly 0.2 or 0.5EDTA
Plasma Renin Activity (PRA)Exactly 0.2 or 0.5EDTA
Renin substrateExactly 0.5 or 1.0EDTA
Angiotensin IIExactly 1.0 or 2.0EDTA
Corticosterone>0.25None or Heparin
Cortisol>0.25None or Heparin
Insulin (human/dog)0.5 or 1.0None
Insulin (rat/mouse)>0.25EDTA, None or Heparin
ACTH>0.25EDTA
Aldosterone>1.0, minimum > 0.5None or Heparin
Leptin (rat/mouse)>0.25EDTA or Serum
Estradial (rat)>0.5Serum or EDTA
Testosterone>0.25None or Heparin