Flow Cytometry and Cell Sorting Core

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Flow Cytometry and Cell Sorting Core Facility

This facility provides flow cytometry analysis, fluorescence activated cell sorting and related services, including consultation for experimental design and data interpretation, to the UMMC research community. A trained operator is available to assist with instrument set-up as well as running and analysis of samples.
 
Before scheduling the first appointment, investigators meet with facility staff to discuss experimental plans to avoid potential pitfalls and optimize the time spent on the machine(s). In addition, a training course consisting of individual help sessions tailored to specific research needs is available upon request.

Instrumentation

The Flow Cytometry Core laboratory has two computer workstations that are for use with the FACSymphony Analyzer and BD FACSAria Fusion cell sorter. Each of these computers has BD FACSDiva acquisition and analysis software, and they also have external hard drives to archive data. The flow core has an addition stand-alone computer that has FlowJo software for data analysis.

BD FACSymphony A3 analyzer BD Biosciences FACSAria Fusion Cell Sorter

Acknowledgement of Core Services:

This Core serves an invaluable resource for many users at UMMC and is supported by multiple NIH funded programs as well as the Department of Physiology & Biophysics. Your use of this core is greatly appreciated and we hope to continue to provide these services to support your research program. Sustainability of these core services is a major goal of the Physiology Department.

As a user of these core services, you are expected to acknowledge the proper funding source that provides support for your use of this core. For every publication, press release or other document regarding above mentioned use of these core services, the appropriate NIH award, Cardiorenal and Metabolic Diseases Research Center P30GM149404 should be cited and acknowledged within PubMed Central (PMC).  Appropriate acknowledgement can be reported as follows: "Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number P30GM149404. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health."

However, if a manuscript includes one or more co-authors whose listed affiliation is with a foreign institution, and the publication acknowledges support from the NIH IDeA Program, including the COBRE award,investigators should contact the COBRE Administrative Core before manuscript submission. NIH has recently emphasized that publications acknowledging IDeA funding and including authors affiliated with foreign institutions may require prior review and approval by NIGMS, even when the research activities were conducted entirely in the United States. The COBRE Administrative Core will help determine whether additional documentation or consultation with NIGMS is needed before the manuscript is submitted or the award is cited.

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