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Hemoglobin Fractionation by HPLC

Test Name:Hemoglobin Fractionation by HPLC
Epic Order Code:LAB5086  
CPT Code:83021  
Specimen(s) Type:Whole Blood, 5.0 mL
Acceptable Container(s):Top is purple (lavender).
Testing Schedule:Monday, Tuesday and Friday, 7 a.m.-3 p.m.
Turn Around Time:STAT: NA  
 Routine: 3-5 days
Collection Information:No special patient preparation is required. Obtain specimen by standard collection procedures.
Transport Information:Deliver to lab immediately. All specimens must be signed into the laboratory.
Reference Clients:If unable to deliver to the lab immediately, store and transport specimen at 2-8° C. Specimen must be received in the lab within 24 hours.
Causes for Rejection:Improperly labeled, incorrect container, contaminated, insufficient quantity, incorrect/delay in transport, grossly hemolyzed or lipemic, clotted.
Reference Range:Hgb A: 96.0 - 100.0%
 Hgb A2: 2.2 - 3.7%
 Hgb C: 0.0%
 Hgb F: 0.0 - 2.0%
 Hgb S: 0.0%
 Other Hgb: 0.0%
 Sickle Cell Screen: Negative
Additional Information:Certain Hemoglobinopathies detected may be confirmed by send out HPLC tests. Hemoglobin A2 may be confirmed by column method and fetal hemoglobin may be confirmed by RID. Blood transfusions may obscure or dilute abnormal Hgb for 3-4 months.