Our laboratory utilizes genetic engineering and dietary manipulations in rodents to investigate the molecular basis of cardiometabolic diseases. Metabolic fluxes and contractile function are assessed simultaneously in the isolated heart perfused in the working mode. Molecular signaling pathways are further interrogated in myocyte cell lines and in primary cultures of isolated cardiac myocytes using molecular cloning and transfection methods followed by transcriptome analysis and immunoblotting. The consequences of the identified molecular alterations for cardiac adaptation to stress conditions (e.g. myocardial ischemia and reperfusion, pressure overload) are then investigated in in vivo models (coronary artery ligation, transverse aortic constriction).