Measurements of blood pressure in rodents. Instrumentation of animals to induce placental ischemia and preeclampsia-like phenotype. Speckle tracking technology and conventional methods for determination of in vivo cardiac structure, function and strain. Measurement of uterine artery resistance. Ex vivo Langendorff and working heart preparations. Use of wire myography to study vascular relaxation and reactivity. Cultured cytotrophoblasts and endothelial cells in addition to migration and invasion assays. Basic biochemical and molecular techniques including ELISA, PCR, immunoblotting, HPLC, protein expression and purification.