The Chade laboratory uses a large animal model of disease (domestic swine). The following list summarizes the most frequently used research methods we employ:
Induction of renovascular hypertension by implantation of a local irritant coil inside the main renal artery, unilaterally or bilaterally, leading to renal artery stenosis, hypertension, and progressive deterioration of renal function and development of renal injury. Induction of dyslipidemia via high-cholesterol diet feeding. Chronic instrumentation for long-term measurement of blood pressure (telemetry). Studies of a single-kidney hemodynamics and function in vivo using high-resolution clinically-validated multi-detector computerized tomography. Studies of the renal microvascular architecture in situ using 3D micro-computerized tomography. Renal histology, morphometric analyses, immunohistochemistry and state of the art molecular biology methods for studying underlying mechanisms of target organ injury in renovascular and chronic renal disease and responses to treatments. Preclinical testing and development of novel therapeutic interventions.