https://www.youtube.com/watch?v=YSieu9rh6KUZippity Doo Dah gives to BCHJackson Free ClinicTelehealth Groundbreaking
  • Mississippi Regional Biophysical Consortium

    Investigators using biophysical techniques in the state of Mississippi have joined together into a user’s consortium to facilitate the sharing of instrumentation and analysis capabilities.

    Provided is a list of members, their affiliation, instrumentation and biophysical expertise. Our goal first and foremost is getting Biophysical science done. By posting Biophysical resources and expertise on the MBC website we hope to facilitate getting experiments done and analyzed. This should enhance the quality and quantity of our research efforts, including undergraduate and graduate studies, and publication of our results in peer reviewed journals.

    This should further be useful in enhancing the Biophysical instrumentation available in the state through improved funding success, especially grants and proposals that involve shared instrumentation. Members listed on this web page agree to share access to equipment and resources and contribute to analysis and discussion of Biophysical data.


    The mission of the Mississippi Regional Biophysical Consortium includes: advertisement and sharing of biophysical instrumentation, topical seminars (advertised on this site), organizing workshops, and facilitating collaborations and grants.

    Disclaimer: Membership in this consortium represents the opportunity for Mississippi colleges and Universities participating in the consortium to share resources (not financial), expertise and collaborators in the general area of biophysics. The parties understand and agree that being a member and listed on this web page does not commit any of the parties to participate in any of the specific activities discussed on this site.

    Affiliated institutions

    University of Mississippi 

     University of Mississippi Medical Center

    Mississippi College

    University of Southern Mississippi

     Belhaven College

    Millsaps College

    Mississippi State University

    University of Alabama in Huntsville


    Board of directors

    Jack Correia, UMMC

    Ed Lewis, MSU

    Charles McCormick, USM

    Randy Wadkins, UM

    Wolfgang Kramer, Millsaps


    Techniques and equipment


    • Analytical ultracentrifugation - UMMC
    • Anton Paar DMA 5000 density meter - UMMC
    • Laser optical tweezers - UMMC
    • Circular Dichroism - Belhaven University, Millsaps College, MSU
    • Dynamic Light Scattering - MSU
    • Differential Scanning Calorimetry - MSU
    • nmr - MSU, UM 
    • Olis Clarity, UMMC

    Meetings of interest


    • Papers published and grants due to MrBC collaborations fostered:


      J. C. Howard, N. I. Hammer, and G. S. Tschumper, "Structures, Energetics and Vibrational Frequency Shifts of Hydrated Pyrimidine," ChemPhysChem, 12, 3262-3273 (2011).

      K. L. Munroe, D. H. Magers, and N. I. Hammer, "Raman Spectroscopic Signatures of Noncovalent Interactions Between Trimethylamine N-oxide (TMAO) and Water," Journal of Physical Chemistry B, 115, 7699-7707 (2011).

      D. N. Reinemann, A. M. Wright, J. D. Wolfe, G. S. Tschumper, and N. I. Hammer, "Vibrational Spectroscopy of N-Methyliminodiacetic Acid (MIDA)-Protected Boronate Ester: Assignment of the B-N Dative Bond Stretching Frequency," Journal of Physical Chemistry B, 115, 6426-6431 (2011). (Cover Article)

      Jamie M. Dettler, Edwin A. Lewis, "Biophysical Studies of the Structure, Stability, and Ligand Binding Properties of G-Quadruplex DNA: Thoughts and Comparisons of the K-ras, c-MYC, and Bcl-2 Oncogene Promoter Sequence Quadruplexes", in Frontiers in Nucleic Acids, American Chemical Society, Washington, DC, pp. 33-50 (2011), doi:  10.1021/bk-2011-1082.ch003


      Joseph P. Emerson, Vu H. Le, Edwin A. Lewis, "Calorimetry"  eLS (Encyclopedia of Life Sciences), 2012, John Wiley & Sons Ltd, http://www.els.net

      He Song, David L. Wilson, Erik R. Farquhar, Edwin A. Lewis, Joseph P. Emerson, "Revisiting Zinc Binding in Human Carbonic Anhydrase" Inorganic Chemistry, 2012 51(20), 11098-105

      Kate L. Henderson, Vu H. Le, Edwin A. Lewis, Joseph P. Emerson, "Exploring Substrate Binding in Homoprotocatechuate 2,3-Dioxygenase using Isothermal Titration  Calorimetry"Journal of Biological Inorganic Chemistry, 2012 17(7), 991-4

      Bidisha Sengupta, Sandipan Chakraborty, Maurice Crawford, Jasmine M. Taylor, Laura E. Blackmon, Pradip K. Biswas, Wolfgang H. Kramer  "Characterization of diadzein-hemoglobin binding using optical spectroscopy and molecular dynamics simulations" Int. J. Biol. Macromol. 2012, 51(3), 250-8.

      Amit Kumar, Lea C. Paslay, Daniel Lyons, Sarah E. Morgan, John J. Correia and Vijayaraghavan Rangacharia, "Specific Soluble Oligomers of Amyloid-β peptide Undergo Replication, and Form Non-Fibrillar Aggregates In Interfacial Environments" (2012) Journal of Biological Chemistry, doi: 10.1074/jbc.M112.355156

      Yu, X., Sigler, S.C., Hossain, D., Wierdl, M., Gwaltney, S.R., Potter, P.M., and Wadkins, R.M., "Global and Local Molecular Dynamics of a Bacterial Carboxylesterase Provide Insight into its Catalytic Mechanism", Journal of Molecular Modeling 18, 2869-2883 (2012). doi: 10.1007/s00894-011-1308-9


      He Song, Andrew C. Weirtz, Michael P. Hendrich, Edwin A. Lewis, Joseph P. Emerson*, "Building reactive copper centers in human carbonic anhydrase II", Journal of Biological Inorganic Chemistry, 2013 18(6), 595-598.

      Ramos, J. P., Le, V. H., Lewis, E. A. (2013), "Role of Water in Netropsin Binding to an A2T2 Hairpin DNA Site: Osmotic Stress Experiments". Journal of Physical Chemistry, Part B,117 (50), 15958-15965.

      Cui, J.; Waltman, P., Le, V. H.,  Lewis, E. A. and (2013), "The Effect of Molecular Crowding on the Stability of Human c-MYC Promoter Sequence i-Motif at Neutral pH". Molecules, 18, 12751-12767.

      Rowland, G.,  B.l Barnett, K.; DuPont, J. I.; Akurathi, G.; Le, V.H., Lewis, E. A. (2013), "The Effect of Pyridyl substitution on the Thermodynamics of Porphyrin Binding to G-quadruplex DNA". Bioorganic & Medicinal Chemistry, 21, 7515-7522

      Machha, V. R., Waddle, J., Turner, A., Wellman, S., Le, V. H., Lewis, E. A. (2013) "Calorimetric Studies of the Interaction of Linker Histone H10 and its Carboxyl (H10-C) and Globular (H10-G) Domains with Calf-Thymus DNA". Biophysical Chemistry, 184, 22-28.

      Le, V.H., Nagesh, N., Lewis, E.A. (2013) "Bcl-2 Promoter Sequence G-Quadruplex Interactions with Three Planar and Non-Planar Cationic Porphyrins, TMPyP4, TMPyP3, and TMPyP2". PLOSONE, 8(8): e72462. DOI:10.1371/journal.pone.0072462 

      Lyons, D.F., Le, V., Bidwell, G.L., Kramer, W.H., Lewis, E.A., Raucher, D., Correia, J.J. (2013) "Structural and Hydrodynamic Analysis of a Novel Drug Delivery Vector: ELP[V5G3A2-150]". Biophysical Journal, 104(9) 2009-2021.

      Siriwardana K., Wang A., Vangala K., Fitzkee N. C., Zhang D. (2013) "Probing the Effects of Cysteine Residues on Protein Adsorption onto Gold Nanoparticles Using Wild-Type and Mutated GB3 Proteins." Langmuir. 29: 10990-10996.


      Le, V. H., Buscaglia, R., Chaires, J. B., Lewis, E. A. (2013), "Modeling Complex Equilibria in ITC Experiments: Thermodynamic Parameters Estimation for a Three Binding Site Model", Analytical Biochemistry, 434(2) 233-241.

      Andrew C. Holley, Keith H. Parsons, Wenming Wan, Daniel F. Lyons, G. Reid Bishop, John J. Correia, Faqing Huang, and Charles L. McCormick, "Block ionomer complexes consisting of siRNA and aRAFT-synthesized hydrophilic-block-cationic copolymers: The influence of cationic block length on gene suppression," Polymer Chemistry, 2014, 5 (24), 6967 - 6976.

      Le, V. H., Yanney, M., McGuire, M., Sygula, A., Lewis, E. A. (2014) "Thermodynamics of Host-Guest Interactions Between Fullerenes and a Buckycatcher". Journal of Physical Chemistry, Part B, 118(41), 11956-11964.

      Zhou, J., Le, V. H., Kalia, D., Nakayamaa, S., Mikek, C., Lewis, E. A., Sintim, H. O. (2014), "Diminazene or berenil, a classic duplex minor groove binder, binds to G-quadruplexes with low nanomolar dissociation constants and the amidine groups are also critical for G-quadruplex binding." Molecular BioSystems. 10,2724-2734.

      Hudson, J. S., Ding, L., Le, V., Lewis, E. A., Graves, D. E. (2014), "Recognition and Binding of Human Telomeric G-Quadruplex DNA by Unfolding Protein 1 (UP1)". Biochemistry, 53(20), 3347-3356

      Lyons, D. F., Le, V., Kramer, W. H., Bidwell, G., Lewis, E. A.; Raucher, D.,Correia, J. J. (2014), "The effect of basic cell penetrating peptides on the structural, thermodynamic and hydrodynamic properties of a novel drug delivery vector: ELP[V5G3A2-150]". Biochemistry, 53(6), 1081-1091.

      Machha, V.R, Jones, S.B., Waddle, J.R., Le, V.H., Wellman, S., Lewis, E.A. (2014), "Exploring the Energetics of Histone H1.1 and H1.4 Duplex DNA Interactions". Biophysical Chemistry, 185, 32-38.

      Reilly, S.M., Lyons, D.F., Wingate, S.E., Wright, R.T., Correia, J.J., Jameson, D.M., and Wadkins, R.M., "Folding and Hydrodynamics of a DNA i-motif from the c-MYC Promoter Determined By Fluorescent Cytidine Analogs", Biophysical Journal107, 1703-1711 (2014). DOI: 10.1016/j.bpj.2014.08.014. PMCID: PMC4190599

      Wang, A., Vo, T., Le, V., Fitzkee, N. C. (2014) "Using Hydrogen-Deuterium Exchange to Monitor Proteins Structure in the Presence of Gold Nanoparticles." Journal of Physical Chemistry B. 118(49): 14148-56.

      Wang, A., Vangala, K. Vo, T., Zhang, D.,Fitzkee, N. C. (2014) "A Three-Step Model for Protein-Gold Nanoparticle Adsorption." Journal of Physical Chemistry C.138: 8134-8142.

      Cuellar, Kristina A.; Munroe, Katherine L.; Magers, David H.; and Hammer, Nathan I. (2014) "Noncovalent Interactions in Microsolvated Networks of Trimethylamine N-Oxide." Journal of Physical Chemistry B118: 449-459.


      Le, V. H., McGuire, M., Ahuja, P., MacDonnell, F., Lewis E. A. (2015) "Thermodynamic investigation of [(phen)2Ru(tatpp)Ru(phen)2]4+ interactions with B-DNA." Journal of Physical Chemistry, Part B, 119(1), 65-71.

      Sengupta, B., Reilly, S.M., Davis, Jr., D., Harris, K., Wadkins, R.M., Ward, D., D'Asia G., Hampton, C., "Excited State Proton Transfer of Natural Flavonoids and Their Chromophores in Duplex and Tetraplex DNAs", Journal of Physical Chemistry B119, 2546-2556 (2015). DOI: 10.1021/jp508599h, PMCID: PMC4329990

      Nettles, W., Song, H., Farquar, E., Fitzkee, N. C., Emerson, J. P. "Characterization of the Copper(II) Binding Sites in Human Carbonic Anhydrase II." (Anticipated 2015) Inorganic Chemistry. In Press.

      Grants received due to MBC:

      From the 2008 meeting: NSF EPSCoR grant EPS-0903787, "Modeling and Simulation of Complex Systems", MBC co-investigators funded include Wadkins, Gwaltney, McCormick, Correia, Hammer, and Magers, 09/01/2009-08/31/2014.

      From the 2008 meeting:  NSF-CRIF award 0840220, "Refurbishment of the 600 MHz NMR at the Mississippi Magnetic Resonance Facility at Mississippi State University", Edwin Lewis (MSU), PI, $510,000 08/01/2009-07/31/2012. The Department of Chemistry at Mississippi State University used this grant to refurbish and cyber enhance a 600 MHz nuclear magnetic resonance (NMR) spectrometer. It is employed in a wide variety of research projects, supporting structural, reaction, and analytical studies. 

      From the 2009 Meeting: NSF MRI-R2 award 0959211, "MRI-R2: Acquisition of Aviv XL-FDS Fluorescence Detection System", John J. Correia (UMC), PI, $438,784 01/01/2010-12/31/2012. This grant allowed for the purchase of an Aviv AU-FDS Fluorescence Detection System. The new system is used to perform hydrodynamic, thermodynamic and macromolecular interaction studies on a wide range of cutting-edge biophysical projects by six major users.

      From the 2013 Meeting: NIH R15 award 1R15GM113152-01, "Functionalized Gold Nanoparticles: Understanding the Mechanism of Protein Binding." Nicholas C. Fitzkee (MSU), PI. $338,814. 01/01/2015-12/31/2018.  This proposal involves heavy utilization of the Bruker 600 MHz spectrometer (NSF-CRF award) listed previously. With the NMR instrumentation, this grant would arguably not have been possible.  In addition, the proposal involves collaborative work with other consortium members to design functionalized nanoconjugates.

      From the 2014 Meeting: NSF EPSCoR Seed Grant Award, "Understanding the Mechanism of Aggregation in Elastin-Like Proteins (ELPs)." Nicholas C. Fitzkee (MSU), PI. John J. Correia (UMC), Co-PI. $36,000, 06/01/2014-05/31/2015. This work came as a direct result of collaboration between consortium members. A future submission to NSF is planned.


       Edwin A. Lewis (PI), Herman O. Sintim (Co-PI), Proposal Title: "G-quadruplex topology selective Probes", Agency: NSF Biology Directorate/Molecular Biophysics Division, Budget: $697,674.