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Flow Cytometry Core


Gallios flow cytometer

The Gallios flow cytometer analyzer offers qualitative and quantitative analysis of cells and other particles using multi-parametric analysis. The instrument is equipped with two solid state lasers, a 488 nm blue laser and a 638 nm red laser and has the ability to simultaneously acquire and distinguish between six different florescent signals in addition to the forward and side scatter parameters allowing for an eight parameter definition of the cell. In addition, the Gallios is optimized for high sensitivity performance and has the capacity to acquire up to 25,000 events per second allowing for a rapid acquisition of samples.

Moflo XDP cell sorter

The MoFlo XDP is a high speed cell sorter with the capacity to simultaneously perform 1 to 4-way sorting. The system is equipped with a 488 nm blue laser, a 355 nm UV laser and a 635 nm red laser. Our current setup can detect two light scatter parameters and six florescent parameters; four on the blue laser, one on the UV laser and one on the red laser making it possible to simultaneously detect and analyze eight different sorting parameters. Samples can be sorted into 12x75 mm FACS tubes or 1.5 ml Eppendorf tubes for 4-way sorts, and into 15 ml centrifugation tubes or 50 ml centrifugation tubes for 2-way sorts. In addition, the MoFlo XDP is equipped with the cyclone sorting option allowing for cells to be directly sorted into 1536-, 384-, 96- or 24-well plates or onto standard slides.

The MoFlo XDP instrument is operated only by the Flow Cytometry facility staff.