Core Facilities


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  • Histology Core Services and Fees

    Histology Core services offered by the Department of Physiology and Biophysics include:

    • General histology: Routine processing, embedding and sectioning of paraffin and frozen tissues. Glycomethacrylate processing and sectioning is available upon request.
    • Special histological staining: A wide array of routine and special histological staining is performed in accordance with established Protocols.
    • Immunohistochemistry: Manual and automated immunohistochemistry services are available for established procedures and protocols. Consultation services are available for developing new protocols for antibodies with unknown specificities.
    • Light microscopy and image analysis: The laboratory offers services and training in assessment of basic histology as well as more complex image analysis (e.g. quantitative analysis of immunohistochemical data, stereology).
    • General training: Training for microtome use, cryosectioning, histological or immunohistochemical staining and image analysis is available at an hourly rate.

    For service fees, please refer to our service fee list.

    We do accept work outside the university which will include a 52.5% surcharge to cover indirect costs.

    Service fees

     Service Charge/Unit
    Processing   

    Paraffin processing (including embedding)

    $1.80/block

    Sectioning   

    Paraffin sectioning (plain slides)

    $1.10/slide

    Paraffin sectioning (adhesive slides)

    $1.70/slide

    Paraffin microtome use

    $10/hour

    Cryosectioning (plain slides)

    $2.00/slide

    Cryosectioning (adhesive slides)

    $2.40/slide

    Cryostat use

    $20/hour

    Serial sectioning (paraffin or cryostat)

    variable

    Staining   

    H&E

    $3.20/slide

    PAS

    $3.15/slide

    Masson's Trichrome

    $4.35/slide

    Picrosirius red

    $2.50/slide

    Oil Red O

    $4.00/slide

    Cresyl violet

    $3.40/slide

    Silver stains

    $4.60/slide

    Other stains by request

    variable

    Immunohistochemistry (primary antibody provided by user)

    $12.50/slide

    Image analysis   

    Digital images (brightfield and fluorescence)

    variable

    Digital microscope use

    $20/hour

    Stereological measurements

    variable

    Other services/consumables   

    Training in paraffin sectioning

    $35.00/hr

    Training in crysectioning

    $35.00/hr

    Training in IHC

    $60.00 procedure

    Training in histological staining

    $15.00/hr

    Plain slides

    $10/box of 50

    Superfrost Plus (+) charged slides

    $20/box of 72

    PBS

    $1.00/L

    Histochoice

    $25/L

    10% buffered formalin

    $2.50/L

    Use of dehydration/rehydration station

    $0.14/slide

    Coverslipping

    $0.36/slide