Core Facilities


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  • Confocal Microscopy

    The Confocal Microscopy facility is located on the second floor (room G272) of the Guyton Research Complex. The Confocal Core houses a Leica TCS-SP2 laser scanning confocal upright microscope. The confocal system has three separate lasers for use with three standard visible length fluorescence dyes as well as transmitted light.

    Services

    The confocal microscope equipped with three individual lasers (488/546/633 nm) capable of imaging standard fluorescence dyes within the visible spectra including FITC/TRITC, CY2/CY3/CY5, green fluorescence protein variants (GFP/YFP/RFP/DsRed) and many different indicator dyes (i.e. Ca 2+, pH, membrane potential, oxidative stress, etc).

    Capabilities

    • Standard confocal microscopy with simultaneous or sequential image collection
    • Line scan, Z-scan modes
    • 10X, 20X, 40X, 40X oil, 60X oil and 100X oil objectives
    • Transmitted light detection
    • Tunable filter
    • Time lapse
    • FRET/FRAP (Fluorescence resonance excitation transfer/ Fluorescence recovery after photobleaching)
    • 3-D reconstruction

    Fees

    • Training: $100/hr
    • User-based imaging: $50/hr

    The Confocal Microscopy Core is primarily an equipment core. Users new to confocal microscopy typically require 2-4 hours of training depending on level of microscopy experience. Experienced users typically require 1-2 hours of training.

    • All users must be trained and pre-approved prior to use by the Confocal Core director.

    Contact information

    • Heather Drummond
             
       Phone: (601) 984-1812
              E-mail: Heather Drummond