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The Confocal Core Facility is located on the 2nd
floor of the new Arthur C. Guyton Research Building in Room G272. The Confocal Core houses a Leica TCS-SP2
laser scanning confocal upright microscope and a Leica TCS-SP8 multiphoton
confocal microscope. Both confocal
systems have 3 separate lasers for use with 3 standard visible length
fluorescence dyes as well as transmitted light.
The multiphoton includes a separate infrared laser.
The SP2 confocal microscope equipped with three individual
lasers (488/546/633 nm) capable of imaging standard fluorescence dyes within
the visible spectra including FITC/TRITC, CY2/CY3/CY5, green fluorescence
protein variants (GFP/YFP/RFP/DsRed) and many different indicator dyes (i.e. Ca2+,
pH, membrane potential, oxidative stress, etc).
This system includes an upright
The SP8 is equipped with three individual lasers
(488/561/633 nm) capable of imaging standard fluorescence dyes within the
visible spectra. This system uses a
light green laser (561) instead of the 546 green laser. The 561 laser can still be used with standard
CY3 and TRITC dyes, but Alexa 555 dyes are ideal. The multiphoton capaibility is provided by a
Coherent sapphire laser. This system
includes an inverted microscope with a Luden chamber.
The Confocal Microscopy Core is primarily an equipment core. Users new to confocal microscopy typically require 2-4 hours of training depending on level of microscopy experience. Experienced users typically require 1-2 hours of training.
2500 North State Street
Jackson, MS 39216
General Information: 601-984-1000
Patient Appointments: 888-815-2005