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Induction of renovascular hypertension by implantation of a local irritant coil inside the main renal artery, unilaterally, leading to renal artery stenosis. Chronic instrumentation for long-term measurement of blood pressure in pigs. Studies of single-kidney hemodynamics and function in vivo using computer tomography. Studies of the renal microvascular architecture in situ using micro-computer tomography. Renal histology, morphmetric analyses, immunohistochemistry and molecular biology methods for studying target organ injury and cytokine expression.
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