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  • Jia L. Zhuo, MD, PhD

     ZhuoJoe_Pharm_000.jpg

    Professor
    Office: G323
    Phone: (601) 984-1707
    Fax: (601) 984-1637
    E-mail: jzhuo@umc.edu

    View NIH Biosketch

    Research interests

    • Angiotensin II receptor pharmacology and signal transduction
    • Renal physiology and pharmacology
    • Hypertension

    Current research

    Data included in AJP cover article, May 2012 (doi: 10.1152/ajpregu.00487.2011)
    Data included in AJP cover article, May 2012 (doi: 10.1152/ajpregu.00487.2011)

     My laboratory is interested in the roles of endocrine (tissue-to-tissue), paracrine (cell-to-cell), and intracrine (cytoplasmic and nuclear) angiotensin II (ANG II) and its G protein-coupled receptor (GPCR) signal mechanisms in the kidney and blood pressure control. Specifically, we are currently working: 1) to determine the molecular and signaling mechanisms by which extracellular (circulating and intrarenally formed) ANG II is taken up by the proximal tubule of the kidney to act as an intracellular peptide, 2) to determine the effects and signaling mechanisms by which internalized or intracellular ANG II induces long-term genomic effects, 3) to determine the role of the sodium and hydrogen exchanger 3 (NHE3) in the proximal tubule on blood pressure responses to extracellular and intracellular ANG II; and 4) the role and signaling mechanisms of ANG II/AT1a receptors in the regulation of urine concentration. To test our hypotheses, we use complementary state of the art approaches including: 1) single proximal tubule cell intracellular microinjection of ANG II, 2) live cell multicolor fluorescent imaging, 3) intravital multi-photon functional imaging of the proximal tubule, 4) proximal tubule cells derived from wild-type or AT1a receptor-deficient (AT1a-KO) mice, 5) global and proximal tubule-specific knockout of AT1a receptors or NHE3, 6) proximal tubule-specific knockin or overexpression of an intracellular cyan fluorescent fusion of ANG II protein (ECFP/ANG II) or a fusion ANG II protein (fsANG II), which is secreted upon expression, 7) high resolution light and electron microscopic autoradiography and immunohistochemistry, and 8) molecular biology and proteomic approaches including real-time and single-cell RT-PCR, gene microarrays, phosphoproteomic  and western blot analyses.

    Selected publications

    • Li, X.C. and Zhuo, J.L. (2013) Proximal tubule-dominant transfer of AT1a receptors induces blood pressure responses to intracellular angiotensin II in AT1a receptor-deficient mice.American journal of physiology, Regulatory, integrative and comparative physiology 304(8), R588-R598. PubMed article
    • Li, X.C. and Zhuo, J.L. (2013)Current insights and new perspectives on the roles of hyperglucagonemia in non-insulin-dependent type 2 diabetes.Current hypertension report 15(5), 522-530.PubMed article
    • Zhuo, J.L., Ferrao, F.M., Zheng, Y. and Li, X.C. (2013) New frontiers in the intrarenal renin-angiotensin system: a critical review of classical and new paradigms.Frontiers in endocrinology (Lausanne). 4:166. PubMed article
    • Zhuo, J.L.,and Li, X.C. (2013) Proximal nephron. Comprehensive physiology3(3),1079-1123.PubMed article
    • Li, X.C., U. Hopfer., and Zhuo, J.L. (2012) Novel signaling mechanisms of intracellular angiotensin II-induced NHE3 expression and activation in mouse proximal tubule cells. American journal of physiology. Renal physiology 303(12), F1617-F1628. PubMed article  
    • Li, X. C., Shao, Y., and Zhuo, J. L. (2012) AT1a receptor signaling is required for basal and water deprivation-induced urine concentration in AT1a receptor-deficient mice, American journal of physiology. Renal physiology. 303:F746-F756. PubMed article
    • Ellis, B., Li, X. C., Miguel-Qin, E., Gu, V., and Zhuo, J. L. (2012) Evidence for a functional intracellular angiotensin system in the proximal tubule of the kidney, American journal of physiology. Regulatory, integrative and comparative physiology 302, R494-509. PubMed article 
    • Zhuo, J. L., and Li, X. C. (2011) New insights and perspectives on intrarenal renin-angiotensin system: focus on intracrine/intracellular angiotensin II, Peptides 32, 1551-1565. PubMed article
    • Li, X. C., and Zhuo, J. L. (2011) Phosphoproteomic analysis of AT1 receptor-mediated signaling responses in proximal tubules of angiotensin II-induced hypertensive rats, Kidney international 80, 620-632. PubMed article
    • Li, X. C., Cook, J. L., Rubera, I., Tauc, M., Zhang, F., and Zhuo, J. L. (2011) Intrarenal transfer of an intracellular fluorescent fusion of angiotensin II selectively in proximal tubules increases blood pressure in rats and mice, American journal of physiology. Renal physiology 300, F1076-1088. PubMed article