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  • Adel Maklad, MD, PhD

     maklad, adel.jpg

    Associate Professor
    Office: R730
    Phone: (601) 984-1656
    Lab: R725; Phone: (601) 984-1684
    Fax: (601) 984-1655
    E-mail: amaklad@umc.edu

      

    Research interest

    I am interested in the role of the epidermal growth factor receptor family in development of cutaneous innervation. I demonstrated that lack of EGFR results in a disorganized pattern of cutaneous innervation, along with a hyperinnervation phenotype. This phenotype is likely a target-derived effect, as revealed by examining the innervation in wild type and mutant skin grafts on nude mice and skin-targeted dominant negative EGFR skin. Microarray analysis of EGFR null and wild type skin suggested that alteration of extracellular matrix molecules such as laminin 5, nidogen, and gelsolin may mediate the EGFR’s effect on sensory nerves of the skin.

    maklad graphic 2We are testing the importance of these matrix molecules for axon outgrowth and branching, using tissue culture model. For this experiment, the dorsal root ganglion cells are cultured on extracellular matrix derived from EGFR wild type and mutant keratinocytes.

    In addition, I am also interested in studying the effect of the hair cell polarity and the gobal polarity of the inner ear on the development of vestibular connectivity pattern to the vestibular nuclear complex and the cerebellum. I am employing a variety of techniques, such as immunocytochemistry and tract tracing. 

     

    Lamprey 3D Figure 1

    A three-dimensional interactive model in which a complete labyrinth of the lamprey is shown; the two ciliated chambers and the semicircular duct system. The model is in Quick Time movie format and can be opened by Quick Time player 7, and can be manipulated in 3 dimensions by dragging with the computer mouse.

    Lamprey 3D Figure 2

    A three-dimensional interactive model of the labyrinth in lamprey in which the semicircular duct system is only shown after digital subtraction of the ciliated chambers. The model is in Quick Time movie format and can be opened by Quick Time player 7, and can be manipulated in 3 dimensions by dragging with the computer mouse.

    Publications

    • Abdel-Malek AK, Saleh MN, Aly YA, Ahmed MG, Maklad A (1995) Brain changes with age on computed tomography. Age & Nutrition 6:93-98.
    • Maklad A, Fritzsch B (1999) Incomplete segregation of endorgan-specific segregation of the vestibular ganglion cells in mice and rat. J Vestib Res 9:387-399.
    • Maklad A, Quinn T, Fritzsch (2001) Intracranial distribution of the sympathetic system in mice: DiI tracing and immunocytochemical labeling. The Anat Rec. 263:99-111.
    • Fritzsch B, Maklad A, Bruce L, Crapon de Caprona, M.-D. (2001) Development of the ear and of the connections between the ear and the brain: the role of microgravity. Adv Space Res 28:595-600.
    • Maklad A, Fritzsch B (2002) The developmental segregation of posterior crista and saccular vestibular fibers in mice: a carbocyanine tracer study using confocal microscopy. Brain Res Dev Brain Res 135:1-17.
    • Fritzsch B, Beisel KW, Jones K, Farinas I, Maklad A, Reichardt L (2002) Development and evolution of inner ear sensory systems and their innervation. J Neurobiol 53: 143-56.
    • Maklad A, Fritzsch B (2003) Partial segregation of posterior crista and saccular fibers to the nodulus and uvula of the cerebellum in mice. Brain Res Dev Brain Re. 140:223-36.
    • Xiang M, Maklad A, Pirvola U, Fritzsch B (2003) Brn3c null mutant mice show long-term, incomplete retention of some afferent inner ear innervation. BMC Neurosci. 4:2
    • Maklad A, Fritzsch B (2003) Development of vestibular afferent projections into hindbrain and their central targets. Brain Res Bull 60:497-510.
    • Maklad A, Fritzsch B, Hansen L (2004) Innervation of the maxillary vibrissae in mice as revealed by anterograde and retrograde tract tracing: Cell Tissue Res 15: 167-180.
    • Beisel KW, Wang-Lundberg Y, Maklad A, Fritzsch B (2005). Development and evolution of the vestibular sensory apparatus of the mammalian ear. J Vestib Res 15: 225-41.
    • Morris JK, Maklad A, Hansen L, Feng Feng, Sorenson C, Lee K, Macklin WB, Fritzsch B (2006) A disorganized innervation of the inner ear persists in the absence of ErbB2. Brain Res (In press).
    • Maklad A, Nicolai J, Bichsel KJ, Evenson JE, Lee T, Threadgill DW, Hansen LA (2008). The EGFR is required for proper innervation to the skin. J Invest Dermatol 120:690-8.
    • Adel Maklad, Suzan Kamel, Elaine Wong, Bernd Fritzsch (2010). Development and Organization of Polarity Specific Segreation of Primary Vestibular Afferent Fibers in Mice. Cell and Tissue Res 340: 303-321.
    • Adel Maklad, Marianne Conway, Cole Hodges, Laura Hansen (2010). Development of Innervation to Maxillary Whiskers in Mice. The Anatomical Record 293: 1553-1567.