Facilities


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  • AUC Director John.J. Correia, PhD
    AUC Director John.J. Correia, PhD

    Analytical Ultracentrifugation Reading List

    XL-A/I reading list

    Introduction

    • K.E. van Holde (1985) Physical Biochemistry, Chap 5, p 110-136
    • Cantor and Schimmel (1980) Biophysical Chemistry, Part II, Chap 11, p 591-641

    Overview of purity and MW measurements

    • D.G. Rhodes and T.M. Laue (1990) Methods Enzy. 182: 555-565
    • T.M. Laue and D.G. Rhodes (1990) Methods Enzy. 182: 566-587

    Boundary analysis

    • K.E. van Holde and W.O. Weischet (1978) Biopoymers 17:1387-1403
    • W.F. Stafford III (1992) Anal. Biochem. 203: 295-301
    • Correia, J. J. Sedimentation velocity data analysis methods: what, when and why? CHEMTRACTS - Biochemistry and Molecular Biology, 11: 944-948, 1998.

    Sedimentation study of small DNA fragments

    • R.T. Kovacic and K.E. van Holde (1977) Biochemistry 16:1490-1498

    Sedimentation velocity of monomer-n-mer system

    • R.P Frigon and S.N. Timasheff (1975) Biochemistry 14:4559-4566
    • R.P Frigon and S.N. Timasheff (1975) Biochemistry 14:4567-4573

    Indefinite self-association, ligand mediated self association

    • Lobert, S., Frankfurter, A., and Correia, J.J. Binding of vinblastine to phosphocellulose-purified and beta-Class III tubulin: The role of nucleotides and beta-tubulin isotypes. Biochemistry, 34: 8050-8060, 1995.
    • Lobert, S., Vulevic, B., and Correia, J.J. Interaction of vinca alkaloids with tubulin: A comparison of vinblastine, vincrisitine, and vinorelbine. Biochemistry, 35: 6806-6814, 1996.
    • Vulevic, B., Lobert, S. and Correia, J.J. Role of Guanine Nucleotides in the Vinblastine-Induced Self-Association of Tubulin: Effects of GMPCPP and GMPCP. Biochemistry 36: 12828-12835, 1997.
    • Correia, J. J. The Analysis of Weight Average Sedimentation Data. Methods in Enzymology, Numerical Computer Methods Part C, 321:81-100, 2000.

    Kirkwood Theory

    Reviews

    • K.E. van Holde (1975) in Proteins Vol 1, Chap. 4 pp. 225-291
    • D.C. Teller (1973) Methods in Enzymol. vol 27D, 346-441

    Sedimentation equilibrium - methods, analysis and examples

    • D.A Yphantis (1964) Biochemistry 3:297-317
    • M.L. Johnson, J.J. Correia, D.A. Yphantis and H.R. Halvorsen (1981) Biophys. J. 36:575-588
    • K.E. Van Holde Fractions 1967 No 1 p 1-10
    • Correia, J.J., Gilbert, S.P., Moyer, M.L. and Johnson, K.A. Sedimentation studies on the Kinesin head domain constructs K401, K366, and K341. Biochemistry 33:4898-4907, 1995.

    Estimation of vbar

    • J.C. Lee and S.N. Timasheff (1974) Biochemistry 13:258-265
    • V. Prakash and S.N. Timasheff (1981) Anal. Biochem. 117:330-335 Done for you in sednterp, available through RASMB

    Solvent equilibration

    • E.F. Casassa and H. Eisenberg (1964) Adv. Protein Chem. 19:287-395

    Drug/ligand binding

    • H.K. Schachman and S.J. Edelstein (1966) Biochemistry 5: 2681-2705
    • I.Z. Steinberg and H.K. Schachman (1966) Biochemistry 5: 3728-3747

    Density/viscosity data

    • International Critical Tables vol III and vol V (Ref Q 199 N274)
    • G. Cohen and H. Eisenberg (1968) Biopolymers 6: 1077-1100.
    • T.M. Laue et al. (1992) Computer-Aided Interpretation of Analytical Sedimentation Data for Proteins pp 90-125 in "Analytical Ultracentrifugation in Biochemistry and Polymer Sciences" (S.E. Harding, A.J. Rowe and J.C. Horton, eds.) Done for you in sednterp, available through RASMB, for a limited number of buffer components.

    Data analysis

    • P.R. Bevington "Data Reduction and Error Analysis for the Physical Sciences"
    • Magar E. Magar "Data Analysis in Biochemistry and Biophysics"
    • J.R. Taylor "An Introduction to Error Analysis"
    • Press et al. "Numerical Recipes" (Fortran & Pascal or C version)
    • M.L. Johnson and S.G. Fraser (1985) Methods in Enzymology 117: 301-342.
    • G. Henderson (1988) J. Chem. Ed. 65: 1001-1003.
    • L. Endrenyi and F.H.F. Kwong (1973) Acta Biol. Med. Germ. 31: 495-505.
    • K.J. Ellis and R.G. Duggleby (1978) Biophys. J. 171: 513-517
    • M.S. Lewis and G.D. Knott (1976) Biophys. Chem. 5: 171-183.
    • "Self-Associating Systems in the Analytical Ultracentrifuge" Beckman