Cancer Institute

  • Molecular Genomics Equipment

    The Molecular and Genomics Facility core occupies approximately 1,100 square feet of laboratory space and is well-equipped to perform genetic and genomic analysis on human and several model organisms, including rat and mouse. The core facility is experienced in all aspects of molecular biology, quantitative RNA analysis and genotyping.

    Equipment includes:

    • General sample preparation and biological storage: Table-top Microfuge (Eppendorf 1524), Temperature Controlled Bench-top Centrifuge (Eppendorf 5810R), Heated Water Baths, Sonicator, Homogenizer, BioRad Chemidoc XRS System, BioTek Flx800 microplate fluorescence reader, Hybaid Maxi-14 hybridization oven, and -80° C Freezer, -20° C Freezer, refrigerators
    • DNA and RNA measurement and quality control: Bio-Rad Experion System, Agilent Bioanalyzer 2100, NanoDrop ND-1000A UV-Vis Spectrophotometer
    • PCR: Two gradient thermocyclers (Bio-Rad myIQ)
    • Sequencing: Beckman CEQ 8000XL GeXP Genetic Analysis System
    • Next-generation sequencing: Illumina® MiSeq™ sequencing instrument. This system provides high-throughput de novo whole genome sequencing of microbial genomes, amplicon sequencing (>1500 amplicons X 96 samples), cancer gene panels, metagenomics, and RNA sequencing (RNAseq).
    • Microarray- whole genome SNP, copy number variation (CNV) and/or gene expression: Affymetrix GeneChip (Scanner 3000 7G System) and GeneAtlas Instruments, Agilent High-Resolution Scanner
    • Validation of microarray, real-time PCR and Taqman® genotyping: Bio-Rad CFX96 Real-Time PCR System, iQ5 BioRad Real-Time PCR Machine
    • DNA analysis and microarray analysis programs: DNAstar (Lasergene), GeneSifter® (Perkin Elmer), Gene Spring (Partek), Ingenuity Pathways Software.